2IDG

Crystal Structure of hypothetical protein AF0160 from Archaeoglobus fulgidus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2006-08-17
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97911
Spacegroup name	P 21 21 21
Unit cell lengths	65.187, 69.744, 133.978
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.830 - 2.690
R-factor	0.24701
Rwork	0.244
R-free	0.29677
Structure solution method	SAD
RMSD bond length	0.012
RMSD bond angle	1.361
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SCA2STRUCTURE
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.800
High resolution limit [Å]	2.690	2.690
Number of reflections	18724
<I/σ(I)>	28.75	4.12
Completeness [%]	97.0	89
Redundancy	11.9	6.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.9	291	SITTING DROP MODIFIED MICROBATCH USING 0.5 MICROLITER DROPS CONTAINING EQUAL VOLUMES OF PROTEIN CONCENTRATE (40 mg/ml) AND RESERVOIR SOLUTION CONTAINING 30% PEG 3350, 0.15M NASCN, 0.01M SPERMINE-HCL, pH 6.9, VAPOR DIFFUSION, SITTING DROP, temperature 291K