2ICR
Red fluorescent protein zRFP574 from Zoanthus sp.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-02-03 |
| Detector | MARRESEARCH |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 115.190, 146.738, 122.928 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.510 |
| Rwork | 0.178 |
| R-free | 0.22500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.033 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | SHELX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.560 |
| High resolution limit [Å] | 1.510 | 1.510 |
| Rmerge | 0.053 | 0.652 |
| Number of reflections | 162291 | |
| <I/σ(I)> | 12.6 | 1.8 |
| Completeness [%] | 99.4 | 94.9 |
| Redundancy | 7.1 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 1.6 M (NH4)2SO4, 0.2 M K/Na TARTRATE, 0.1 M TRIS-HCL, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
