2ICG

Crystal structure of a protein of unknown function (NP_472245.1) from Listeria innocua at 1.65 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2006-08-11
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.94926,0.97925
Spacegroup name	P 63 2 2
Unit cell lengths	100.030, 100.030, 62.740
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.501 - 1.650
R-factor	0.2
Rwork	0.198
R-free	0.23500
Structure solution method	MAD
RMSD bond length	0.018
RMSD bond angle	1.526
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELX
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.501		1.710
High resolution limit [Å]	1.650	2.820	1.650
Rmerge	0.094	0.073	0.011
Number of reflections	22770
<I/σ(I)>	9.89	21.4	1.35
Completeness [%]	99.6	99.6	97.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		9	277	1.6M (NH4)2SO4, 0.1M Bicine, pH 9.0, VAPOR DIFFUSION, SITTING DROP, NANODROP, temperature 277K