2IC9
The Coiled-coil Domain (residues 1-93) Structure of the Sin Nombre Virus Nucleocapsid Protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-C |
| Synchrotron site | APS |
| Beamline | 14-BM-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-04-12 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9002 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 85.774, 42.647, 57.996 |
| Unit cell angles | 90.00, 121.11, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.000 |
| R-factor | 0.257 |
| Rwork | 0.247 |
| R-free | 0.32800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ic6 chain A |
| RMSD bond length | 0.029 |
| RMSD bond angle | 2.444 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.039 | 0.248 |
| Number of reflections | 12165 | |
| <I/σ(I)> | 32.7 | 7.5 |
| Completeness [%] | 98.8 | 99 |
| Redundancy | 4 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.15 M potassium bromide, 0.1 M Tris-HCl, 34% PEG mono-methyl ether (PEG MME) 200, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
