2IBN
Crystal structure of Human myo-Inositol Oxygenase (MIOX)
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-08-26 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9876 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 119.715, 55.861, 111.516 |
| Unit cell angles | 90.00, 116.72, 90.00 |
Refinement procedure
| Resolution | 29.340 - 1.500 |
| R-factor | 0.208 |
| Rwork | 0.206 |
| R-free | 0.24800 |
| Structure solution method | SAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.522 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.600 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Number of reflections | 140563 | |
| <I/σ(I)> | 12.21 | 3.1 |
| Completeness [%] | 67.9 | 27 |
| Redundancy | 4.6 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 291 | 100 mM Tris pH 7.8, 1.8 M Ammonium sulphate, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
