2IBB

Crystal Structure of the First and Second FNIII Domains of Ihog

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-BM-C
Synchrotron site	APS
Beamline	14-BM-C
Detector technology	CCD
Collection date	2004-06-29
Detector	ADSC QUANTUM 315
Wavelength(s)	0.900
Spacegroup name	P 21 21 21
Unit cell lengths	39.380, 65.240, 84.210
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	21.570 - 2.400
Rwork	0.244
R-free	0.27500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	First FNIII domain Ihog and PDB Entry: 1FNF domain 7
RMSD bond length	0.008
RMSD bond angle	1.665
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.490
High resolution limit [Å]	2.400	2.400
Rmerge	0.126	0.801
Number of reflections	8851
<I/σ(I)>	7.5
Completeness [%]	98.1	99.3
Redundancy	5	5.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.9	293	0.05M Tris-HCl pH 7.9, 23% PEG 3350, 0.2M ammonium sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	7.9	293	0.05M Tris-HCl pH 7.9, 23% PEG 3350, 0.2M ammonium sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 293K