2IAZ
Crystal structure of a Conserved Protein of Unknown Function SP1372 from Streptococcus pneumoniae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-12-05 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97980 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 64.808, 86.715, 93.881 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.630 - 2.400 |
| R-factor | 0.23188 |
| Rwork | 0.229 |
| R-free | 0.28902 |
| Structure solution method | SAD |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.646 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.630 | 2.462 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.082 | 0.366 |
| Number of reflections | 20102 | |
| <I/σ(I)> | 20.2 | 3 |
| Completeness [%] | 99.5 | 99.48 |
| Redundancy | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | 60% Tacsimate, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
