2IAH
Crystal structure of the ferripyoverdine receptor of the outer membrane of Pseudomonas aeruginosa bound to ferripyoverdine.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-09-05 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.97857 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 82.123, 82.123, 286.225 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.960 - 2.730 |
| R-factor | 0.24611 |
| Rwork | 0.244 |
| R-free | 0.28522 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1xkh |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.096 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.960 | 2.880 |
| High resolution limit [Å] | 2.730 | 2.730 |
| Number of reflections | 24636 | |
| <I/σ(I)> | 7.3 | 1.3 |
| Completeness [%] | 94.4 | 78.3 |
| Redundancy | 2.9 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 298 | Na Acetate 0.1 M, pH 4.6; 12 mM MgSO4, 12 % PEG 3350. Protein concentration in 0.5 % C8E4: 15 mg/ml, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
