2IAF
Crystal structure of a fragment (residues 11 to 161) of L-serine dehydratase from Legionella pneumophila
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Detector technology | CCD |
Collection date | 2006-08-15 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9793, 0.97943 |
Spacegroup name | I 21 21 21 |
Unit cell lengths | 56.319, 76.835, 108.135 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.000 - 2.050 |
R-factor | 0.19602 |
Rwork | 0.195 |
R-free | 0.22418 |
Structure solution method | MAD |
RMSD bond length | 0.020 |
RMSD bond angle | 2.009 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | HKL-3000 |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.090 |
High resolution limit [Å] | 2.050 | 2.050 |
Rmerge | 0.078 | 0.478 |
Number of reflections | 14934 | |
<I/σ(I)> | 17 | |
Completeness [%] | 99.1 | 100 |
Redundancy | 7.1 | 5.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 0.2 Ammonium sulphate, 0.1M BisTris pH 6.5, 25% Peg 3350, VAPOR DIFFUSION, HANGING DROP, temperature 291K |