2IAD

CLASS II MHC I-AD IN COMPLEX WITH AN INFLUENZA HEMAGGLUTININ PEPTIDE 126-138

Experimental procedure

Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL7-1
Synchrotron site	SSRL
Beamline	BL7-1
Temperature [K]	95
Detector technology	IMAGE PLATE
Collection date	1997-04
Detector	MARRESEARCH
Spacegroup name	C 1 2 1
Unit cell lengths	127.200, 100.200, 53.100
Unit cell angles	90.00, 100.30, 90.00

Refinement procedure

Resolution	24.000 - 2.400
R-factor	0.253
Rwork	0.253
R-free	0.30800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1dlh
RMSD bond length	0.009
RMSD bond angle	29.700 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	X-PLOR (3.8)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	24.000	2.500
High resolution limit [Å]	2.400	2.400
Rmerge	0.076	0.259
Number of reflections	22555	2820 *
Completeness [%]	89.0	88
Redundancy	2.5	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	7.4	22 *	19% PEG 8000 0.2 M TRIS, PH 7.4

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	5-9 (mg/ml)
2	1	reservoir	PEG8000	19 (%)
3	1	reservoir	Tris	0.2 (M)