2IAA
Crystal Structure of an Electron Transfer Complex Between Aromatic Amine Dephydrogenase and Azurin from Alcaligenes Faecalis (Form 2)
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-06-07 |
| Detector | SBC |
| Wavelength(s) | 1.381, 1.445 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 67.216, 131.722, 133.204 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.040 - 1.950 |
| R-factor | 0.174 |
| Rwork | 0.174 |
| R-free | 0.20500 |
| Structure solution method | MAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.400 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.040 | |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.059 | 0.202 |
| Number of reflections | 80127 | |
| <I/σ(I)> | 14.8 | 3.4 |
| Completeness [%] | 78.0 | 68.6 |
| Redundancy | 2.6 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 295 | PEG, MES and calcium acetate, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
