2IA9
Structural Genomics, the crystal structure of SpoVG from Bacillus subtilis subsp. subtilis str. 168
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-08-03 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97711 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 136.464, 136.464, 110.493 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.950 - 3.000 |
| R-factor | 0.23014 |
| Rwork | 0.227 |
| R-free | 0.29600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2i9x |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.602 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.950 | 3.070 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.135 | 0.506 |
| Number of reflections | 21472 | |
| <I/σ(I)> | 13.6 | 3.48 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 5.5 | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 277 | 0.2M Ammonium sulfate, 0.1M Sodium citrate, 15%(w/v)PEG4000, pH 5.6, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
