2IA1
Crystal structure of protein BH3703 from Bacillus halodurans, Pfam DUF600
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-07-30 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 71.385, 81.448, 91.135 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 60.750 - 1.590 |
| R-factor | 0.173 |
| Rwork | 0.172 |
| R-free | 0.19200 |
| Structure solution method | SAD |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.569 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELX |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.750 | 1.660 |
| High resolution limit [Å] | 1.590 | 1.590 |
| Rmerge | 0.042 | 0.695 |
| Number of reflections | 71141 | |
| <I/σ(I)> | 13.2 | 2.84 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 7.8 | 7.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298 | 2.0 M (NH4)2SO4, Tris, 0.2 M LiSO4, 10% Ethylene Glycol, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
