2I9Z
Structural Genomics, the Crystal structure of full-length SpoVG from Staphylococcus epidermidis ATCC 12228
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-07-09 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97932 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 63.604, 63.604, 98.498 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.990 - 2.300 |
| R-factor | 0.22533 |
| Rwork | 0.223 |
| R-free | 0.26408 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2i9x |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.421 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.990 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.129 | 0.531 |
| Number of reflections | 9486 | |
| <I/σ(I)> | 18.98 | 2.54 |
| Completeness [%] | 99.7 | 97.7 |
| Redundancy | 7.2 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 277 | 0.2M Ammonium sulfate, 0.1M Sodium acetate, 12%(w/v) PEG4000 , pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
