2I9X
Structural Genomics, the crystal structure of SpoVG conserved domain from Staphylococcus epidermidis ATCC 12228
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-06-02 |
| Detector | SBC-3 |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 64.550, 64.550, 97.711 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.850 - 1.800 |
| R-factor | 0.18981 |
| Rwork | 0.188 |
| R-free | 0.22260 |
| Structure solution method | SAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.491 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.510 | 1.840 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.063 | 0.459 |
| Number of reflections | 19791 | |
| <I/σ(I)> | 31.62 | 3.73 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 6.1 | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 5%(w/v) isopropanol, 0.1M HEPES pH 7.5, 10%(v/w) PEG4000, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
