2I9T
Structure of NF-kB p65-p50 heterodimer bound to PRDII element of B-interferon promoter
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 1999-02-08 |
| Detector | BRANDEIS - B4 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 97.620, 166.110, 60.050 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.460 - 2.800 |
| R-factor | 0.233 |
| Rwork | 0.233 |
| R-free | 0.28800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.400 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.064 | 0.337 |
| Number of reflections | 24691 | |
| <I/σ(I)> | 27.87 | 4.4 |
| Completeness [%] | 99.4 | 96.5 |
| Redundancy | 3.68 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.6 | 293 | 8% PEG 5K monomethylether, 200mM sodium acetate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K, pH 5.60 |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | sodium acetate | ||
| 2 | 1 | 1 | PEG 5K monomethylether | ||
| 3 | 1 | 1 | HOH | ||
| 4 | 1 | 2 | sodium acetate | ||
| 5 | 1 | 2 | PEG 5K monomethylether | ||
| 6 | 1 | 2 | HOH |
