2I91
60kDa Ro autoantigen in complex with a fragment of misfolded RNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 120 |
| Detector technology | CCD |
| Collection date | 2005-04-25 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 72.680, 119.980, 73.540 |
| Unit cell angles | 90.00, 98.91, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.650 |
| R-factor | 0.245 |
| Rwork | 0.241 |
| R-free | 0.28500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1yvp |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.626 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.740 |
| High resolution limit [Å] | 2.650 | 2.650 |
| Rmerge | 0.130 | 0.398 |
| Number of reflections | 34410 | |
| <I/σ(I)> | 12 | 3.9 |
| Completeness [%] | 96.5 | 99.8 |
| Redundancy | 3.3 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 298 | 18-20% PEG 6000, 250 mM magnesium acetate, 100 mM sodium acetate Protein was mixed with RNA approximately 1:1., pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 6000 | ||
| 2 | 1 | 1 | magnesium acetate | ||
| 3 | 1 | 1 | sodium acetate | ||
| 4 | 1 | 1 | H2O | ||
| 5 | 1 | 2 | PEG 6000 | ||
| 6 | 1 | 2 | magnesium acetate | ||
| 7 | 1 | 2 | sodium acetate |
