2I88
Crystal structure of the Channel-forming Domain of Colicin E1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 295 |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU RAXIS II |
| Spacegroup name | I 4 |
| Unit cell lengths | 87.460, 87.460, 59.100 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.000 - 2.500 |
| R-factor | 0.176 |
| Rwork | 0.172 |
| R-free | 0.28400 |
| Structure solution method | MIR |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.600 |
| Data reduction software | DENZO |
| Data scaling software | CCP4 ((ROTAVATA)) |
| Phasing software | CCP4 |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.000 | 2.640 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.125 | |
| Number of reflections | 6684 | |
| <I/σ(I)> | 13.97 | 5.5 |
| Completeness [%] | 86.5 | 61.4 |
| Redundancy | 3.7 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 295 | 50 mM MES at pH 6.0 25 mM Tris-HCl pH 7-8 100 mM NaCo or NaNO3 23-27% PEG 3350 very sensitive to temperatue stabilized by slow addition to of PEG 20,000 to 2-3%, VAPOR DIFFUSION, temperature 295K |
