2I7F
Sphingomonas yanoikuyae B1 ferredoxin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X6A |
| Synchrotron site | NSLS |
| Beamline | X6A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-02-21 |
| Wavelength(s) | 1.03320 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 62.064, 62.064, 238.436 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 9.500 - 1.900 |
| R-factor | 0.196 |
| Rwork | 0.193 |
| R-free | 0.24000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fqt |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.935 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK (9.1L) |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.000 | 1.680 |
| High resolution limit [Å] | 1.620 | 1.620 |
| Rmerge | 0.125 | 0.539 |
| Total number of observations | 315 | |
| Number of reflections | 25261 | |
| <I/σ(I)> | 8.1 | 1.2 |
| Completeness [%] | 70.4 | 7.2 |
| Redundancy | 4.74 | 1.24 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 288 | 0.05M phosphate buffer, 0.1M citric acid, 1.6M ammonium sulfate, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 288K |
