2I7D
Structure of Human cytosolic deoxyribonucleotidase in complex with deoxyuridine, AlF4 and Mg2+
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-09-01 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.8133 |
| Spacegroup name | P 1 |
| Unit cell lengths | 39.623, 47.153, 62.384 |
| Unit cell angles | 68.92, 81.69, 74.99 |
Refinement procedure
| Resolution | 19.100 - 1.200 |
| R-factor | 0.166 |
| Rwork | 0.166 |
| R-free | 0.19600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.463 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | CNS |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.120 | 1.300 |
| High resolution limit [Å] | 1.200 | 1.200 |
| Rmerge | 0.067 | 0.357 |
| Number of reflections | 120303 | |
| <I/σ(I)> | 10.24 | 3.2 |
| Completeness [%] | 93.1 | 86.8 |
| Redundancy | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 298 | 50mM KH2PO4, 2-15% w/v PEG 8000, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
