2I7A
Domain IV of Human Calpain 13
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-08-18 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.54178 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 86.115, 86.115, 47.417 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.000 - 1.800 |
| R-factor | 0.17054 |
| Rwork | 0.169 |
| R-free | 0.19699 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1kfu |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.496 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 18860 | |
| <I/σ(I)> | 33.18 | 1.2 |
| Completeness [%] | 99.0 | 89.6 |
| Redundancy | 9.6 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 298 | 25% PEG4K, 0.2M NH4SO4, 0.1M Na-Ac, 10 mg/mL protein solution, cryoprotected with 25% glycerol, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
