2I75

Crystal Structure of Human Protein Tyrosine Phosphatase N4 (PTPN4)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	CCD
Collection date	2006-07-12
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.979
Spacegroup name	P 41 21 2
Unit cell lengths	66.075, 66.075, 144.508
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.920 - 2.450
R-factor	0.22671
Rwork	0.224
R-free	0.29104
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2b49
RMSD bond length	0.012
RMSD bond angle	1.347
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	PHASER
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.920	2.580
High resolution limit [Å]	2.450	2.450
Number of reflections	11901
Completeness [%]	95.6	98.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.8	293	0.1M HEPES, 0.1 M NaCl, 1.3 M (NH4)SO4. , pH 6.8, VAPOR DIFFUSION, SITTING DROP, temperature 293K