2I6O
Crystal structure of the complex of the archaeal sulfolobus PTP-fold phosphatase with phosphopeptides N-G-(p)Y-K-N
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-07-31 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 80.979, 58.399, 36.435 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.900 |
| Rwork | 0.191 |
| R-free | 0.22660 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ohe |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.238 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.090 | 0.607 |
| Number of reflections | 12980 | |
| <I/σ(I)> | 17.5 | 2.47 |
| Completeness [%] | 91.4 | 92.6 |
| Redundancy | 5.1 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
