2I5U
Crystal structure of DnaD domain protein from Enterococcus faecalis. Structural genomics target APC85179
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-04-26 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97835 |
| Spacegroup name | P 43 |
| Unit cell lengths | 48.795, 48.795, 32.795 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.500 - 1.500 |
| R-factor | 0.18175 |
| Rwork | 0.180 |
| R-free | 0.22702 |
| Structure solution method | SAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.020 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.550 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.071 | 0.422 |
| Number of reflections | 22919 | |
| <I/σ(I)> | 32.68 | 2.13 |
| Completeness [%] | 97.3 | 88.5 |
| Redundancy | 8.8 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 298 | 0.2M Magnesium chloride, 25% PEG3350, pH 5.5, temperature 298K, VAPOR DIFFUSION, HANGING DROP |
