2I5T

Crystal Structure of hypothetical protein LOC79017 from Homo sapiens

Experimental procedure

Experimental method	SAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2006-06-18
Detector	MARMOSAIC 300 mm CCD
Spacegroup name	P 21 21 21
Unit cell lengths	35.052, 100.138, 102.498
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.620 - 2.010
R-factor	0.186
Rwork	0.183
R-free	0.23400
Structure solution method	SAD
RMSD bond length	0.020
RMSD bond angle	1.506
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.621	2.080
High resolution limit [Å]	2.010	2.010
Rmerge	0.123	0.357
Number of reflections	24297
<I/σ(I)>	12.185	4.096
Completeness [%]	97.5	83.2
Redundancy	10.8	8.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	PROTEIN SOLUTION (10 MG/ML PROTEIN, 0.050 M SODIUM CHLORIDE, 0.003 M SODIUM AZIDE, 0.0003 M TCEP, 0.005 M BISTRIS PH 7.0) MIXED IN A 1:1 RATIO WITH THE WELL SOLUTION (28% PEG 2K, 0.20 M POTASSIUM GLUTAMATE, 0.1 M MES/ACETATE PH 5.5) CRYOPROTECTED WITH 10% ETHYLENE GLYCOL IN WELL SOLUTION, VAPOR DIFFUSION, HANGING DROP, temperature 293K