2I5L

Crystal structure of Bacillus subtilis Cold Shock Protein variant Bs-CspB M1R/E3K/K65I

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.2
Synchrotron site	BESSY
Beamline	14.2
Temperature [K]	110
Detector technology	CCD
Collection date	2005-07-18
Detector	MAR CCD 165 mm
Wavelength(s)	0.9537
Spacegroup name	P 43 21 2
Unit cell lengths	55.500, 55.500, 55.470
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	18.500 - 2.550
Rwork	0.227
R-free	0.24400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1csq
RMSD bond length	0.016
RMSD bond angle	1.717
Data reduction software	XDS (VERSION June 2005)
Data scaling software	XSCALE
Phasing software	AMoRE
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.200	2.600
High resolution limit [Å]	2.500	2.500
Rmerge	0.088	0.580
Number of reflections	3223
<I/σ(I)>	20.14	2.8
Completeness [%]	98.2	98.6
Redundancy	9.72	9.36

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	293.15	protein solution: 20 mM TRIS pH 7.5, 50 mM sodium chloride, 3 mM magnesium chloride, 20.4 mg/ml protein. crystallization buffer: 25 % PEG 3350, 0.2 M sodium carbonate, 0.1 M TRIS HCl pH 8.5. crystallization setup: 0.8 microliter protein solution:0.8 microliter reservoir solution, VAPOR DIFFUSION, SITTING DROP, temperature 293.15K