2I5I
CRYSTAL STRUCTURE OF A PUTATIVE CELLOBIOSE-PHOSPHATE CLEAVAGE PROTEIN (EF3048) FROM ENTEROCOCCUS FAECALIS V583 AT 1.70 A RESOLUTION
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-05-07 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.918370,0.978981,0.979291 |
| Spacegroup name | H 3 |
| Unit cell lengths | 130.530, 130.530, 85.760 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.450 - 1.700 |
| R-factor | 0.146 |
| Rwork | 0.144 |
| R-free | 0.18500 |
| Structure solution method | MAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.024 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | SHELX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 29.450 | 50.000 | 1.740 |
| High resolution limit [Å] | 1.700 | 4.100 | 1.700 |
| Rmerge | 0.080 | 0.052 | 0.505 |
| Number of reflections | 59414 | ||
| <I/σ(I)> | 20.8 | 2 | |
| Completeness [%] | 99.6 | 98.1 | 100 |
| Redundancy | 3.6 | 3.5 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION,SITTING DROP,NANODROP | 7 | 277 | 40.0% MPD, 0.1M HEPES pH 7.0, VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 277K |
