2I5E
Crystal Structure of a Protein of Unknown Function MM2497 from Methanosarcina mazei Go1, Probable Nucleotidyltransferase
Experimental procedure
| Experimental method | SAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-07-03 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97929 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 43.797, 100.482, 54.877 |
| Unit cell angles | 90.00, 106.15, 90.00 |
Refinement procedure
| Resolution | 38.810 - 2.100 |
| R-factor | 0.19461 |
| Rwork | 0.192 |
| R-free | 0.24866 |
| Structure solution method | SAD |
| RMSD bond length | 0.029 |
| RMSD bond angle | 2.492 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.810 | 2.150 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.079 | 0.383 |
| Number of reflections | 25854 | |
| <I/σ(I)> | 19.72 | 2.52 |
| Completeness [%] | 97.0 | 77.3 |
| Redundancy | 4.7 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 277 | 35% PEG3350, 0.1M Tris, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
