2I4S
PDZ domain of EpsC from Vibrio cholerae, residues 204-305
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Collection date | 2005-08-10 |
| Wavelength(s) | 0.979124 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 111.244, 25.499, 87.654 |
| Unit cell angles | 90.00, 125.38, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.920 |
| R-factor | 0.179 |
| Rwork | 0.176 |
| R-free | 0.23600 |
| Structure solution method | SAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.403 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.010 |
| High resolution limit [Å] | 1.940 | 4.180 | 1.940 |
| Rmerge | 0.093 | 0.070 | 0.278 |
| Number of reflections | 15285 | ||
| <I/σ(I)> | 7 | ||
| Completeness [%] | 97.7 | 97.8 | 89.4 |
| Redundancy | 3.3 | 3 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 10 | 294 | 2.4M ammonium sulfate, 0.2M Li sulfate, 0.1M CAPS pH 10.0, vapor diffusion, temperature 294K |
| 1 | VAPOR DIFFUSION | 10 | 294 | 2.4M ammonium sulfate, 0.2M Li sulfate, 0.1M CAPS pH 10.0, vapor diffusion, temperature 294K |
