2I4L
Rhodopseudomonas palustris prolyl-tRNA synthetase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-1 |
Synchrotron site | ESRF |
Beamline | ID14-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-02-28 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.934 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 110.840, 212.560, 150.560 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | ? - 2.000 |
R-factor | 0.19241 |
Rwork | 0.191 |
R-free | 0.22124 |
Structure solution method | MAD followed by molecular replacement |
RMSD bond length | 0.015 |
RMSD bond angle | 1.416 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | SHELXD |
Refinement software | REFMAC (5.2.0000) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.030 |
High resolution limit [Å] | 1.960 | 1.960 |
Number of reflections | 116008 | |
Completeness [%] | 91.3 | 56.5 |
Redundancy | 5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 0.1 M citric acid (pH 5.5), 15-17 % PEG 3000, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 0.1 M citric acid (pH 5.5), 15-17 % PEG 3000, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 0.1 M citric acid (pH 5.5), 15-17 % PEG 3000, VAPOR DIFFUSION, HANGING DROP, temperature 293K |