2I4I

Crystal Structure of human DEAD-box RNA helicase DDX3X

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	CCD
Collection date	2006-05-22
Detector	ADSC QUANTUM 315
Wavelength(s)	1.072
Spacegroup name	P 31 2 1
Unit cell lengths	67.288, 67.288, 253.470
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	14.910 - 2.200
R-factor	0.18873
Rwork	0.187
R-free	0.21822
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1WRB for the DEAD domain and 2DB3 for the helicase domain
RMSD bond length	0.017
RMSD bond angle	1.584
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	14.910	2.260
High resolution limit [Å]	2.200	2.200
Number of reflections	32841
Completeness [%]	99.2	98.7
Redundancy	10.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.5	277	1.75M Na formate, 0.1M Tris pH 7.5, 20mM ATPgS and MgCl2, VAPOR DIFFUSION, temperature 277K