2I49
Crystal structure of apo form of Bicarbonate Transport Protein CmpA from Synechocystis sp. PCC 6803
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-05-10 |
| Detector | Bruker Platinum 135 |
| Spacegroup name | P 1 |
| Unit cell lengths | 44.660, 49.680, 57.570 |
| Unit cell angles | 87.57, 81.20, 75.20 |
Refinement procedure
| Resolution | 25.600 - 1.350 |
| R-factor | 0.178 |
| Rwork | 0.178 |
| R-free | 0.19300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ID 2I48 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.346 |
| Data reduction software | SAINT |
| Data scaling software | PROTEUM PLUS (PLUS) |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.600 | 1.450 |
| High resolution limit [Å] | 1.350 | 1.350 |
| Number of reflections | 103049 | |
| <I/σ(I)> | 29.8 | 6 |
| Completeness [%] | 99.5 | 98.1 |
| Redundancy | 7.2 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | batch/microseeding | 8 | 298 | Ammonium sulfate, HEPPS pH 8.0, EGTA, batch/microseeding, temperature 298K |
