2I45
Crystal structure of protein NMB1881 from Neisseria meningitidis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-02-04 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97935 |
| Spacegroup name | P 43 |
| Unit cell lengths | 94.932, 94.932, 155.704 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.500 |
| R-factor | 0.20245 |
| Rwork | 0.199 |
| R-free | 0.26793 |
| Structure solution method | SAD |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.260 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.520 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.095 | 0.455 |
| Number of reflections | 46681 | |
| <I/σ(I)> | 21.9 | 1.4 |
| Completeness [%] | 98.5 | 82.3 |
| Redundancy | 9.8 | 8.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9.5 | 289 | K/Na Tartrate, CHES, Lithium sulfate, pH 9.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
