2I3S
Bub3 complex with Bub1 GLEBS motif
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 120 |
| Detector technology | CCD |
| Collection date | 2005-08-15 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97780 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.656, 79.181, 142.582 |
| Unit cell angles | 90.00, 98.34, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.900 |
| R-factor | 0.221 |
| Rwork | 0.219 |
| R-free | 0.26200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1u4c |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.350 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 79711 | |
| Completeness [%] | 90.6 | 68.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.4 | 296 | 18% PEG 3K 200 mM NaCl 100 mM Cacodylate, pH 6.4, VAPOR DIFFUSION, HANGING DROP, temperature 296K |
