2I3P
K28R mutant of Homing Endonuclease I-CreI
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-08-11 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 43.076, 68.211, 87.409 |
| Unit cell angles | 90.00, 91.42, 90.00 |
Refinement procedure
| Resolution | 27.690 - 2.300 |
| Rwork | 0.234 |
| R-free | 0.29200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1g9y |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | EPMR (2.5) |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.690 | 2.340 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.039 | 0.097 |
| Number of reflections | 21742 | |
| <I/σ(I)> | 26.2 | |
| Completeness [%] | 96.0 | 93.9 |
| Redundancy | 3.2 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | 298 | PEG 400 28%, pH 6.5, VAPOR DIFFUSION, temperature 298K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 400 | ||
| 2 | 1 | 1 | HOH | ||
| 3 | 1 | 2 | PEG 400 | ||
| 4 | 1 | 2 | HOH |
