2I3O
Crystal structure of gamma-glutamyl transferase related protein from Thermoplasma acidophilum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-06-14 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 116.080, 95.471, 119.040 |
| Unit cell angles | 90.00, 109.79, 90.00 |
Refinement procedure
| Resolution | 30.470 - 2.030 |
| R-factor | 0.183 |
| Rwork | 0.183 |
| R-free | 0.20700 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELX |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.100 |
| High resolution limit [Å] | 2.030 | 2.030 |
| Rmerge | 0.095 | |
| Number of reflections | 156657 | |
| <I/σ(I)> | 11.5 | 3 |
| Completeness [%] | 99.6 | 97.9 |
| Redundancy | 7.4 | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | Magnesium Formate, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
