2I37
Crystal structure of a photoactivated rhodopsin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-11-13 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.97620 |
| Spacegroup name | P 31 1 2 |
| Unit cell lengths | 161.245, 161.245, 143.442 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 4.150 |
| R-factor | 0.378 |
| Rwork | 0.377 |
| R-free | 0.38200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2i36 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.069 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 4.300 |
| High resolution limit [Å] | 4.150 | 4.150 |
| Rmerge | 0.063 | 0.860 |
| Number of reflections | 12498 | |
| <I/σ(I)> | 11.1 | |
| Completeness [%] | 76.8 | 61.2 |
| Redundancy | 8.9 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.3 | 278 | 80 mM MES, pH 6.3, 110 mM N-nonyl-beta-D-glucoside, 200 mM zinc acetate, 0.1% sodium azide, 16 mM beta-mercaptoethanol, 2.6% Merpol HCS, equilibrated against 3.1-3.3 M ammonium sulfate, VAPOR DIFFUSION, temperature 278K |
