2I30
Human serum albumin complexed with myristate and salicylic acid
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS FR591 |
| Temperature [K] | 293 |
| Detector technology | CCD |
| Collection date | 2004-08-20 |
| Detector | BRUKER SMART 2000 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 187.661, 38.879, 95.515 |
| Unit cell angles | 90.00, 105.50, 90.00 |
Refinement procedure
| Resolution | 45.700 - 2.900 |
| Rwork | 0.195 |
| R-free | 0.25100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1bj5 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.593 |
| Data reduction software | PROTEUM PLUS (PLUS) |
| Data scaling software | PROTEUM PLUS (PLUS) |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.700 | 3.080 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Number of reflections | 13859 | |
| Completeness [%] | 91.0 | 88.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION, vapor diffusion, sitting drop | 7.5 | 293 | 31% PEG 3350, 0.05M potassium phosphate, pH 7.5, EVAPORATION, vapor diffusion, sitting drop, temperature 293K |
