2I2R
Crystal structure of the KChIP1/Kv4.3 T1 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-03-06 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.116 |
| Spacegroup name | P 1 |
| Unit cell lengths | 93.151, 98.105, 97.783 |
| Unit cell angles | 91.00, 112.56, 111.77 |
Refinement procedure
| Resolution | 91.290 - 3.350 |
| R-factor | 0.23807 |
| Rwork | 0.237 |
| R-free | 0.26811 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1S1G (For Kv4.3 T1 domain) PDB entry 1S6C (For KChIP1) |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.415 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 91.290 | 3.540 |
| High resolution limit [Å] | 3.350 | 3.350 |
| Number of reflections | 40483 | |
| <I/σ(I)> | 8.2 | 1.7 |
| Completeness [%] | 96.9 | 96.9 |
| Redundancy | 2.2 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 296 | 15% PEG3000, 0.2M sodium chloride, 0.1M Bis-Tris, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 296K |
