2I25

Crystal structure analysis of the nurse shark New antigen Receptor PBLA8 variable domain in complex with lysozyme

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2005-05-07
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.95369
Spacegroup name	P 21 21 21
Unit cell lengths	73.979, 82.719, 107.363
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.710 - 1.800
R-factor	0.18664
Rwork	0.185
R-free	0.21083
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.021
RMSD bond angle	1.762
Phasing software	AMoRE
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.710	1.850
High resolution limit [Å]	1.800	1.800
Number of reflections	60320
<I/σ(I)>	19.7	1.7
Completeness [%]	98.2	100
Redundancy	4.2	4.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.3	295	38% PEG 400, 0.1M Tris, pH 8.3, VAPOR DIFFUSION, SITTING DROP, temperature 295K