2I24
Crystal structure analysis of the nurse shark New Antigen Receptor PBLA8 variable domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-01-12 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.97623 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 53.958, 53.958, 79.077 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.700 - 1.350 |
| R-factor | 0.1741 |
| Rwork | 0.172 |
| R-free | 0.21430 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1sq2 CHAIN N |
| RMSD bond length | 0.022 |
| RMSD bond angle | 1.802 |
| Phasing software | EPMR |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.700 | 1.370 |
| High resolution limit [Å] | 1.350 | 1.350 |
| Number of reflections | 26060 | |
| <I/σ(I)> | 16.7 | 1.9 |
| Completeness [%] | 98.7 | 95.6 |
| Redundancy | 3.8 | 1.95 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 277 | 24% PEG 400, 0.1M Na/K phosphate, 0.15M NaCl, pH 7.4, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
