2I21
Bacteriorhodopsin/lipid complex, T46V mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-04-15 |
| Detector | ADSC QUANTUM |
| Wavelength(s) | 0.97977 |
| Spacegroup name | P 63 |
| Unit cell lengths | 61.069, 61.069, 110.534 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 25.000 - 1.840 |
| R-factor | 0.1965 |
| Rwork | 0.195 |
| R-free | 0.25960 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1c3w |
| RMSD bond length | 0.015 |
| RMSD bond angle | 0.054 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELX |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 1.920 |
| High resolution limit [Å] | 1.840 | 1.840 |
| Rmerge | 0.041 | 0.537 |
| Number of reflections | 19393 | |
| <I/σ(I)> | 28.9 | 4.1 |
| Completeness [%] | 95.5 | 90.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | CUBIC LIPID PHASE | 5.6 | 295 | MO:WATER:PHOSPHATE, pH 5.6, CUBIC LIPID PHASE, temperature 295K |
