2I1K
Moesin from Spodoptera frugiperda reveals the coiled-coil domain at 3.0 angstrom resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 112 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.000 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 126.940, 126.940, 272.517 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 3.000 |
| R-factor | 0.184 |
| Rwork | 0.181 |
| R-free | 0.24900 |
| Structure solution method | Refinement against 2.1 angstrom structure |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.929 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 3.110 |
| High resolution limit [Å] | 3.000 | 6.460 | 3.000 |
| Rmerge | 0.144 | 0.051 | 0.437 |
| Number of reflections | 17202 | ||
| <I/σ(I)> | 5.6 | ||
| Completeness [%] | 99.3 | 98.6 | 99.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | 100 mM HEPES pH 8.0, 15% PEG 8000, 50 mM phosphoserine, 800 mM NaCl, 1 M urea. Crystals were soaked in harvesting solution containing 1 mM IP3 for 2 hours prior to data collection, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
