2I1A

A Retroviral Protease-Like Domain in the Eukaryotic Protein Ddi1

Experimental procedure

Experimental method	MAD
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	120
Detector technology	IMAGE PLATE
Collection date	2005-04-29
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418, 0.97935, 0.97949, 0.91165
Spacegroup name	P 21 21 21
Unit cell lengths	46.800, 74.330, 153.540
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.960 - 2.300
Rwork	0.229
R-free	0.26600
Structure solution method	MAD
RMSD bond length	0.007
RMSD bond angle	1.342
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.960	2.380
High resolution limit [Å]	2.300	2.300
Number of reflections	24391
<I/σ(I)>	18.8	3.9
Completeness [%]	98.8	90
Redundancy	7.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.4	293	10 mM potassium phosphate, 1% dioxane, 12.5-22.5% PEG2000, pH 7.4, VAPOR DIFFUSION, temperature 293K