2I10
Putative TetR transcriptional regulator from Rhodococcus sp. RHA1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-02-06 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97935 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 44.709, 53.554, 178.946 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.800 - 2.050 |
| R-factor | 0.2058 |
| Rwork | 0.202 |
| R-free | 0.24520 |
| Structure solution method | SAD |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.507 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.800 | 2.110 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmerge | 0.083 | 0.603 |
| Number of reflections | 26965 | |
| <I/σ(I)> | 38.3 | 2.44 |
| Completeness [%] | 96.7 | 77.9 |
| Redundancy | 13.3 | 8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 0.2 M Ammonium sulfate, 0.1 M Hepes buffer, 25% PEG 3350, 50 mM p-nitrophenol, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
