2I0M
Crystal structure of the phosphate transport system regulatory protein PhoU from Streptococcus pneumoniae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-08-08 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97980 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 72.547, 88.965, 75.790 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.720 - 2.400 |
| R-factor | 0.23746 |
| Rwork | 0.235 |
| R-free | 0.28624 |
| Structure solution method | SAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.444 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 56.250 | 2.460 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.100 | 0.470 |
| Number of reflections | 9296 | |
| <I/σ(I)> | 17.76 | 2.97 |
| Completeness [%] | 98.7 | 99.15 |
| Redundancy | 8.8 | 8.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298 | 0.01M Zinc Sulfate, 0.1M MES, 25% W/V PEG MME550, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
