2I0K
Cholesterol Oxidase from Brevibacterium sterolicum- His121Ala Mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.98 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 140.635, 85.851, 78.755 |
| Unit cell angles | 90.00, 112.48, 90.00 |
Refinement procedure
| Resolution | 72.550 - 1.600 |
| R-factor | 0.14888 |
| Rwork | 0.148 |
| R-free | 0.16724 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1i19 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.231 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 72.550 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.094 | 0.549 |
| Number of reflections | 109450 | |
| <I/σ(I)> | 18.06 | 5.07 |
| Completeness [%] | 96.1 | 95.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.2 | 290 | PEG 8K, MnSO4, NaCacodylate, pH 5.2, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
