2I06
Escherichia Coli Replication Terminator Protein (Tus) Complexed With DNA- Locked form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-03-02 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97929 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 62.834, 62.834, 252.454 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.200 |
| R-factor | 0.22098 |
| Rwork | 0.218 |
| R-free | 0.28032 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ewj |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.960 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.072 | 0.175 |
| Number of reflections | 17442 | |
| <I/σ(I)> | 25.52 | 4.04 |
| Completeness [%] | 64.6 | 13.1 |
| Redundancy | 5.1 | 1.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.75 | 298 | 13% PEG 3350, 50mM Bis-Tris pH6.75, 200mM NaI, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 3350 | ||
| 2 | 1 | 1 | sodium iodide | ||
| 3 | 1 | 1 | Bis-Tris | ||
| 4 | 1 | 1 | water | ||
| 5 | 1 | 2 | PEG 3350 | ||
| 6 | 1 | 2 | sodium iodide |
