2HZF
Crystal structures of a poxviral glutaredoxin in the oxidized and reduced states show redox-correlated structural changes
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-01-14 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.1 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 61.976, 67.573, 108.553 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.760 - 1.800 |
| R-factor | 0.18208 |
| Rwork | 0.180 |
| R-free | 0.21916 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.022 |
| RMSD bond angle | 1.772 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Refinement software | REFMAC (5.2.0001) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.200 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 20896 | |
| Completeness [%] | 96.7 | 88 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 295 | 20 % MPD, 0.1 M NaCacodylate pH 6.0, 5 mM DTT, 10 mM Tris pH 8.0, 100 mM sodium chloride, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
