2HZ7
Crystal structure of the Glutaminyl-tRNA synthetase from Deinococcus radiodurans
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 160 |
| Detector technology | CCD |
| Collection date | 2004-05-20 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.934 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 74.100, 95.900, 115.700 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.670 - 2.300 |
| R-factor | 0.2 |
| Rwork | 0.200 |
| R-free | 0.24200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1o0b |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.500 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.670 | 2.360 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.062 | 0.250 |
| Number of reflections | 36991 | |
| <I/σ(I)> | 18.1 | 5.5 |
| Completeness [%] | 98.1 | 92.9 |
| Redundancy | 5.7 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 7.5 | 298 | 4 mg/ml protein, 10% PEG 3350 and 0.1 M NaF (final concentrations) in microbatch under paraffin oil, pH 7.5, Microbatch, temperature 298K |
